TESA — Testicular Sperm Aspiration for Male Infertility | ART Fertility Clinics

TESA (Testicular Sperm Aspiration) is a sperm retrieval procedure where sperm are extracted directly from testicular tissue using a fine needle under local anaesthesia. At ART Fertility Clinics, TESA is recommended for men with obstructive azoospermia — including those who have had a vasectomy or have congenital absence of the vas deferens. Retrieved sperm are combined with ICSI to achieve fertilisation rates comparable to ejaculated sperm in most cases.

reviewed Medically reviewed by Dr. Richa Jagtap , Medical Director & Senior IVF Specialist - ART Fertility Clinics, India — Last reviewed: July 2026
Testicular Sperm Aspiration (TESA)
64%

IVF Success Rate

TESA Procedure for Male Infertility: Everything you Need to Know

Are you suffering from male infertility that reduces the likelihood of becoming a parent?

TESA, which stands for Testicular Sperm Aspiration, might be the solution. It’s a reliable way to extract sperm from the testicle, particularly if sperm is missing in the ejaculate.

At ART Fertility Clinics, we offer couples access to the latest in scientific and science-based technologies and compassionate care in creating their family.

What is TESA?

TESA means Testicular Sperm Aspiration. It is a surgical process where the sperm are extracted straight from the testicle with a very fine needle.

It is commonly suggested for men whose semen contains no sperm, a condition called azoospermia. This can happen due to:

  • Blockages in the reproductive tract
  • Genetic conditions
  • Hormonal problems
  • Unsuccessful ejaculate because of injury or disease

TESA is often coupled with IVF (In-Vitro Fertilization) or ICSI (Intracytoplasmic Sperm Injection). ‘It allows couples to carry on, even with the most difficult fertility cases.

When is TESA Recommended?

TESA is often suggested when:

  • There is no sperm in the ejaculated semen (azoospermia)
  • A vasectomy or other obstruction of the sperm has occurred in the past.
  • There are very few sperm, and they need to be directly retrieved
  • A couple is undergoing ICSI, and high-quality sperm is needed
  • There are issues with ejaculation due to spinal cord injury or other causes

Male infertility accounts for almost 50% of infertility worldwide as per the World Health Organization (WHO) statistics. There is hope for such couple, procedures such as TESA give hope.

How is TESA procedure performed?

TESA is an office procedure performed under local anesthesia. You can get this done with no side effect and it's very fast, safe 100% guaranteed. Here’s how the process works:

  • The skin above the testicle is cleaned and given anesthetic
  • With a fine needle, a tiny sample of testicular tissue is withdrawn
  • This tissue is eventually sent to the embryology laboratory in order to check for live sperm
  • If present, the sperm is immediately used for ICSI or cryopreserved for later use
  • The complete process will typically last 15 to 30 minutes. There is no cutting or sewing

Is TESA Painful?

TESA is described by most men as feeling like a pinprick. There may be mild discomfort or swelling afterward, but it generally dissipates in a day or two. Rest, cool packs and mild pain relivers are generally sufficient.

TESA vs Other Techniques of Sperm Extraction

TESA is a type of surgical sperm retrieval. These involve TESE (Testicular Sperm Extraction) and Micro-TESE. Here's a quick comparison:

TESA vs Other Techniques
Technique Method When Used
TESA Needle aspiration First-line choice in many azoospermia cases
TESE Small tissue biopsy If TESA does not retrieve sperm
Micro-TESE Microsurgical biopsy For very low sperm production

TESA is generally less invasive and preferred for the initial attempt.

TESA with IVF/ICSI

The sperm obtained by TESA are usually immotile (not moving) and are unable to fertilize an egg on their own. That is why it is combined with ICSI, where a single sperm is injected directly into the egg.

Fertilization rate and pregnancy rates significantly increases with a combination of TESA and ICSI. Combined approach is particularly beneficial for couples with male infertility.

TESA Success Rate

In the case of TESA, success is being able to recover/extract good sperm. The outcome is influenced by the etiology of azoospermia.

  • In obstructive azoospermia (due to blockage), sperm retrieval can be as successful as 90 to 100%.
  • In non-obstructive azoospermia (low or no sperm produced), 40–60% are successful with sperm being produced.
  • After the retrieval and use of sperm in ICSI, the probability of pregnancy depends on different factors:
  • Quality of the sperm
  • Age and fertility status of the female partner
  • Shape of the eggs and embryos

What Happens After the Procedure?

Recovery from TESA is quick. Most men are back to a regular routine within a day or two. Some may feel:

  • Mild pain or discomfort
  • Slight swelling or bruising
  • Sensitivity at the site
  • To aid healing, doctors generally recommend:
  • Light activity for a day
  • Abstinence for a couple of days
  • No lifting or gym for a week or two

Your doctor will tell you when you can get back to your usual activities.

Is TESA Safe?

Sure, TESA is safe and low-risk procedure when performed by experienced operator. Some rare side effects are:

  • Minor bleeding
  • Infection (very rare)
  • Temporary testicular discomfort

At ART Fertility Clinics, we have all safety precautions in place pre, during and post treatment for optimum results and patient comfort.

Why to choose ART Fertility Clinic for TESA?

We believe that hopeful parents of every kind deserve a scientific, caring, and personalized path to parenthood. Our team includes:

  • Expert andrologists and embryologists
  • State of the art labs with latest sperm analysis and storage capabilities
  • Rigorous quality control and ethical treatment
  • Good outcomes with TESA-ICSI cycles
  • Encouraging, people to walk you through it.

We know fertility treatment can be emotional. That’s why we pair science, diplomacy and care to smooth the route along the way.

Conclusion

Male infertility isn’t the end of the line. With increasing options such as TESA, many men can be biological dads, even if there are no sperm in their ejaculate.

TESA is safe, efficacious, and well established as a minimally invasive procedure. With ICSI it provides hope and results for countless couples suffering from male factor infertility.

If you are considering fertility, contact us at ART Fertility Clinics. We’re here for you to navigate forward, with care, clarity and confidence.

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IVF and Infertility Specialist Dr. Richa Jagtap, Dr. Manjushri Amol Kothekar, Dr. Pankush Gupta
Dr. Richa Jagtap

Dr. Richa Jagtap

MBBS, DGO MD (Obst & Gynae.)

Medical Director & Senior IVF Specialist - ART Fertility Clinics, India

24+ Yrs Exp
star rating 5.0/5
Dr. Manjushri Amol Kothekar

Dr. Manjushri Amol Kothekar

Master Of Surgery (Obst & Gynae.)

Senior Consultant - ART Fertility Clinics, Mumbai & Navi Mumbai

15+ Yrs Exp
star rating 5.0/5
Dr. Pankush Gupta

Dr. Pankush Gupta

MBBS , MD (OBG)

Sr. Consultant - ART Fertility Clinics, Faridabad

12+ Yrs Exp
star rating 5.0/5

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How Testicular Sperm Aspiration (TESA) Works

TESA retrieves sperm directly from the testicle using a fine needle under local anaesthesia — a quick day-case procedure for men with obstructive azoospermia.

01

Consultation & Assessment

Your specialist evaluates your history and semen results to confirm TESA is the appropriate approach.

02

Local Anaesthesia

A local anaesthetic numbs the area so the procedure can be performed comfortably as a day case.

03

Testicular Aspiration

A fine needle is inserted into the testicle to aspirate a small amount of tissue and fluid containing sperm.

04

Sample Examination

The sample is assessed in the laboratory to confirm the presence of viable sperm.

05

Sperm Use or Freezing

Retrieved sperm is used directly for ICSI or cryopreserved for a future cycle.

06

Recovery

Recovery is quick, with most patients resuming normal activities within a day.

Not sure which treatment is right for you?

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People Also Asked

TESA is a minor surgical procedure used to retrieve sperm directly from the testes in men who have low or no sperm count in their ejaculate. A fine needle is inserted into the testicle to collect sperm, which is then used for IVF or ICSI treatments.

TESA is recommended for men with obstructive azoospermia (blocked sperm ducts), non-obstructive azoospermia, or failed sperm production in ejaculate. It’s often used when conventional sperm retrieval methods are not effective.

TESA has a high success rate in men with obstructive azoospermia (up to 90%). For non-obstructive cases, success rates are around 50–60%, depending on testicular function and overall health.

TESA is performed under local anesthesia, so discomfort is minimal. Some men may experience swelling, bruising, or mild pain afterward. Rare risks include infection, bleeding, or damage to testicular tissue.

The cost of TESA in India typically ranges from ₹25,000 to ₹60,000, depending on the fertility clinic, technique, and whether IVF or ICSI is performed alongside.

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